LLM Mart Basic

Run full computational-pathology workflows with PathML — whole-slide-image (WSI) analysis across 160+ slide formats, multiplexed immunofluorescence (CODEX, Vectra, MERFISH), nucleus segmentation/classification (HoVer-Net, HACTNet), tissue- and cell-graph construction, HDF5 datase

Build phylogenetic trees end-to-end from raw sequences — MAFFT multiple sequence alignment, optional TrimAl trimming, IQ-TREE 3 maximum-likelihood inference with model selection and bootstraps, FastTree for large datasets, then visualize with ETE3 or FigTree. Use when reconstruct

Design protein sequences for a fixed backbone with ProteinMPNN (Dauparas 2022) — message-passing inverse folding that outputs sequences predicted to fold to a given structure, with fixed positions, tied/symmetric chains, amino-acid bias, and a soluble-model variant. Use when inve

Run differential gene expression analysis on bulk RNA-seq count matrices with PyDESeq2, the Python port of DESeq2 — size-factor normalization, dispersion estimation, Wald tests, FDR (Benjamini-Hochberg) correction, and volcano/MA plots. Use when identifying differentially express

Build complete mass-spectrometry workflows with pyOpenMS — feature detection, peptide identification, protein quantification, and full LC-MS/MS pipelines across many MS file formats (mzML, mzXML) and algorithms. Use for comprehensive proteomics and MS data processing — for simple

Read and write genomic alignment and variant files in Python with pysam (htslib bindings) — SAM/BAM/CRAM alignments, VCF/BCF variants, and FASTA/FASTQ sequences, plus region extraction and per-base coverage/pileup. Use when scripting NGS data-processing pipelines that parse, filt

Runs 16S/ITS amplicon (microbiome) analysis with the QIIME 2 distribution (2026.7; the "amplicon" distribution was renamed "qiime2" in 2026.4) in the correct order: manifest import, cutadapt trim-paired primer removal BEFORE dada2 denoise-paired (trunc-len chosen from the demux q

Generate de-novo protein backbones with RFdiffusion (Watson 2023) — a diffusion model for unconditional monomer generation, motif scaffolding, binder design against a target, and symmetric oligomers. Use when generating a new protein backbone from scratch, scaffolding a functiona

Quantifies bulk RNA-seq transcript abundance with salmon 2.x (the Rust rewrite; selective alignment or --sketch) and kallisto (v0.52.0, kb-python workflow), builds a decoy-aware gentrome index, runs quant with --gcBias -l A, then imports estimates via tximport/tximeta with a tx2g

Run the standard single-cell RNA-seq analysis pipeline with Scanpy on AnnData — QC filtering, normalization, dimensionality reduction (PCA, UMAP, t-SNE), Leiden/Louvain clustering, marker/differential expression, PAGA trajectories, and plotting. Use when analyzing scRNA-seq data

Apply the scGPT single-cell foundation model (Cui 2024) to annotate and embed cells — zero-shot and fine-tuned cell-type annotation, gene/cell embeddings, batch integration, and gene-regulatory / perturbation inference from AnnData. Use when annotating cell types with a pretraine

Run RNA velocity analysis with scVelo on single-cell RNA-seq data — estimate cell-state transitions from spliced/unspliced mRNA dynamics, infer trajectory direction, compute latent time, and identify driver genes. Use when adding directionality to trajectories or studying differe

Train deep generative models for single-cell omics with scvi-tools — probabilistic batch correction and integration (scVI), reference-mapping transfer learning (scArches), differential expression with uncertainty, and multimodal models (totalVI for CITE-seq, MultiVI for multiome)

Analyzes spatial transcriptomics with squidpy (1.8.x) on AnnData and SpatialData objects, routing platforms correctly: Visium spots use spatial_neighbors(coord_type='grid') and pair with deconvolution, while Xenium/MERFISH single-cell data use coord_type='generic'/Delaunay neighb

Store and query genomic variant data at scale with TileDB-VCF — ingest VCF/BCF into compressed TileDB arrays, add samples incrementally, run fast parallel region/sample queries, and export back to VCF. Use when managing population-genomics variant datasets that are too large for

Wraps RDKit in a high-level, pandas-friendly datamol interface with sensible defaults for everyday drug discovery — SMILES/SDF loading into DataFrames, molecule standardization, descriptors, fingerprints, Butina clustering, 3D conformer generation, scaffold analysis, and parallel

Runs molecular machine learning with DeepChem — diverse featurizers, pre-built MoleculeNet benchmark datasets, and pre-trained models (ChemBERTa, GROVER) for property prediction (ADMET, toxicity, solubility) via traditional ML or graph neural networks. Use when running end-to-end

Predicts protein-ligand binding poses with DiffDock diffusion-based molecular docking from PDB structures and SMILES, producing pose confidence scores for virtual screening and structure-based drug design. Use when docking ligands into a protein, generating binding poses, or scre

Computes mass-spectral similarity and identifies compounds for metabolomics with matchms — comparing mass spectra, scoring similarity (cosine, modified cosine), and searching spectral libraries to annotate unknowns. Use when matching MS/MS spectra, identifying metabolites, or lib

Applies medicinal-chemistry filters with the medchem library — drug-likeness rules (Lipinski, Veber), PAINS filters, structural alerts, and molecular complexity metrics for compound prioritization and library cleanup. Use when filtering or triaging a compound library, flagging PA

Fourteen posts of being wrong in production, compressed to checkboxes

Healthy nodes, a quiet network, 300 restarts in three days, and a latency budget measured in milliseconds

Discovery worked. Ping worked. Every TCP connection timed out, and later the tunnel only worked when someone had a terminal open.

Every VM came back. The cluster did not. Declarative systems converge on config, and the datapath isn't config.

A surprising share of AI-in-the-terminal failures aren't the AI. They're zsh, and a version of bash from 2006.

A Claude Code plugin turns standalone project configuration into a namespaced, installable extension that teams and communities can update as one unit.

None of the safety came from the model. It came from six boring habits.

Skills package instructions and references. Subagents run work in a separate context and return results. They solve different problems and can be composed deliberately.

Six hours in, one step left, everything green, and the incident that didn't happen

CLAUDE.md carries persistent project context. Skills load reusable procedures when relevant. Separating stable facts from task-specific workflows keeps both easier to maintain.

Twenty minutes recovering secrets that never existed, and the one sentence from a human that ended it

An API request routing a model's tool call through an approval gate to a remote MCP server

31 config keys, two audits, and why the first one was wrong in both directions

The official MCP Registry stores standardized server metadata rather than package code. Publishers verify a namespace, describe installation or remote access, and submit immutable versions.

Everyone looks at the Dockerfile. The file that actually leaked the key was the project file.

Remote MCP authorization uses established OAuth standards, but secure integration still requires issuer validation, least-privilege scopes, protected token handling, and server-side enforcement.

"Copy it over and switch the reference" is two steps, and the outage lives in the one nobody checks

stdio fits local processes and prototypes. Streamable HTTP fits hosted services and shared integrations. The right choice follows where the capability runs and who must reach it.

The most important rule wasn't about what I could change. It was about what I was allowed to display.

Tools perform operations, resources expose readable context, and prompts provide reusable templates. Choosing the correct primitive makes an MCP server easier to understand and govern.

Universal System Design Philosophy - the 17 core principles, short for /ar:philosophy

Plan export status and settings [on|off|globalon|globaloff|dir <path>|pattern <template>|rejected [on|off|dir <path>]|reset]

Create a structured plan, short for /ar:plannew

Execute an approved plan step by step, short for /ar:planprocess

Critique and improve an existing plan, short for /ar:planrefine

Start autoproc - procedural autonomous workflow

Sync an existing plan with the codebase, short for /ar:planupdate

Force-reload all integration rules from config files

Restart the daemon for the current autorun install/source tree

Start autorun - autonomous task execution

Emergency stop - immediately halt all actions (short for /ar:estop)

Show current AutoFile policy status (short for /ar:status)

Show current AutoFile policy and settings

Graceful stop - finish current task then stop autorun

Inspect tasks or configure pause, prompts, recovery, and ignore behavior

Plural alias for task status, pause, prompts, recovery, and ignore

Verify that the autorun plugin command surface is loaded

Tmux session management - create, list, cleanup isolated sessions, short for /ar:tmux

Tmux session management - create, list, cleanup isolated sessions

CLI testing in isolated tmux sessions, short for /ar:ttest

Open-source, self-hosted AI media server. One server replaces your entire media stack, with a web app, native iPhone app, and an AI agent that gets things done.…

A curated list of tools built for Jev — TypeSafe AI's System One model for typed decisions.

🦦 Crayotter: A Multimodal AI-Agent for Video-Editing, Video-Composing, and Video Production. Powered by Multimodal LLMs for autonomous Text-to-Video agentic fr…

Webextension tool for Odoo

基于多模态视觉感知与 LLM Agent 的 macOS 微信自动化框架 | Visual RPA for WeChat

The operational superset of Pi Coding Agent — everything Pi, plus observability, governance, recovery, evaluation and multi-agent orchestration. Pi Coding Agent…

Metrik 可以集中查看本机多个 Agent 的配额余量和 Token 消耗，目前支持 ChatGPT、Claude、GLM、Kimi 等主流 AI 服务。

An AI agent with a real self — soul she wrote, desires that drive her, a heartbeat for autonomous action, dreams she processes when you're away. Capability supe…

Production-ready open source terminal coding agent with readable, layered code: permission rules, OS sandboxing, MCP, skills, sub-agents, and Anthropic, OpenAI-…

Answer me with HTML — an agent skill that answers hard questions with a one-page HTML you can actually read. 让 AI Agent 用一页 HTML 回答复杂问题。

Prompt packs that make any AI agent a LaTeX expert — fix errors, polish writing, format for venues, read papers, recover source

Claude Code plugin: universal radial-tree exploration engine. One tree skill + swappable presets (brainstorm / attack / design / code-audit) for divergent ideat…

Claude Code + OpenClaw + Codex + WorkBuddy 中文教程 | 50篇完整教程 + 1张速查卡 | 80万+内容量 | 1500+实操示例 | AI Coding / Agent 四线学习路径（编程+助手+Agent+办公）

SmartLabelBench 2026: LLM-Powered Auto Annotation and Dataset Builder for Everything

从零开始玩转OpenClaw：最全面的中文教程，涵盖安装、配置、实战案例和避坑指南（github版）

Native Android GUI for running AI coding agents locally on-device. No terminal or PC required.

基于 LangChain/LangGraph 的 ReAct Agent ，结合 RAG、工具调用与 Streamlit 界面，面向智能客服与报告生成场景。

An open-source, local-first AI learning workbench

Open-source coding agent for your terminal, built in Rust and on a journey of continuous community improvement. Issues and PRs welcome.

Matt Pocock 技能集的中文翻译版 — 地道中文，原汁原味的技术术语。基于 mattpocock/skills 复刻。每日中午12点钟同步