LLM Mart Basic

Run differential gene expression analysis on bulk RNA-seq count matrices with PyDESeq2, the Python port of DESeq2 — size-factor normalization, dispersion estimation, Wald tests, FDR (Benjamini-Hochberg) correction, and volcano/MA plots. Use when identifying differentially express

Build complete mass-spectrometry workflows with pyOpenMS — feature detection, peptide identification, protein quantification, and full LC-MS/MS pipelines across many MS file formats (mzML, mzXML) and algorithms. Use for comprehensive proteomics and MS data processing — for simple

Read and write genomic alignment and variant files in Python with pysam (htslib bindings) — SAM/BAM/CRAM alignments, VCF/BCF variants, and FASTA/FASTQ sequences, plus region extraction and per-base coverage/pileup. Use when scripting NGS data-processing pipelines that parse, filt

Runs 16S/ITS amplicon (microbiome) analysis with the QIIME 2 distribution (2026.7; the "amplicon" distribution was renamed "qiime2" in 2026.4) in the correct order: manifest import, cutadapt trim-paired primer removal BEFORE dada2 denoise-paired (trunc-len chosen from the demux q

Generate de-novo protein backbones with RFdiffusion (Watson 2023) — a diffusion model for unconditional monomer generation, motif scaffolding, binder design against a target, and symmetric oligomers. Use when generating a new protein backbone from scratch, scaffolding a functiona

Quantifies bulk RNA-seq transcript abundance with salmon 2.x (the Rust rewrite; selective alignment or --sketch) and kallisto (v0.52.0, kb-python workflow), builds a decoy-aware gentrome index, runs quant with --gcBias -l A, then imports estimates via tximport/tximeta with a tx2g

Run the standard single-cell RNA-seq analysis pipeline with Scanpy on AnnData — QC filtering, normalization, dimensionality reduction (PCA, UMAP, t-SNE), Leiden/Louvain clustering, marker/differential expression, PAGA trajectories, and plotting. Use when analyzing scRNA-seq data

Apply the scGPT single-cell foundation model (Cui 2024) to annotate and embed cells — zero-shot and fine-tuned cell-type annotation, gene/cell embeddings, batch integration, and gene-regulatory / perturbation inference from AnnData. Use when annotating cell types with a pretraine

Run RNA velocity analysis with scVelo on single-cell RNA-seq data — estimate cell-state transitions from spliced/unspliced mRNA dynamics, infer trajectory direction, compute latent time, and identify driver genes. Use when adding directionality to trajectories or studying differe

Train deep generative models for single-cell omics with scvi-tools — probabilistic batch correction and integration (scVI), reference-mapping transfer learning (scArches), differential expression with uncertainty, and multimodal models (totalVI for CITE-seq, MultiVI for multiome)

Analyzes spatial transcriptomics with squidpy (1.8.x) on AnnData and SpatialData objects, routing platforms correctly: Visium spots use spatial_neighbors(coord_type='grid') and pair with deconvolution, while Xenium/MERFISH single-cell data use coord_type='generic'/Delaunay neighb

Store and query genomic variant data at scale with TileDB-VCF — ingest VCF/BCF into compressed TileDB arrays, add samples incrementally, run fast parallel region/sample queries, and export back to VCF. Use when managing population-genomics variant datasets that are too large for

Wraps RDKit in a high-level, pandas-friendly datamol interface with sensible defaults for everyday drug discovery — SMILES/SDF loading into DataFrames, molecule standardization, descriptors, fingerprints, Butina clustering, 3D conformer generation, scaffold analysis, and parallel

Runs molecular machine learning with DeepChem — diverse featurizers, pre-built MoleculeNet benchmark datasets, and pre-trained models (ChemBERTa, GROVER) for property prediction (ADMET, toxicity, solubility) via traditional ML or graph neural networks. Use when running end-to-end

Predicts protein-ligand binding poses with DiffDock diffusion-based molecular docking from PDB structures and SMILES, producing pose confidence scores for virtual screening and structure-based drug design. Use when docking ligands into a protein, generating binding poses, or scre

Computes mass-spectral similarity and identifies compounds for metabolomics with matchms — comparing mass spectra, scoring similarity (cosine, modified cosine), and searching spectral libraries to annotate unknowns. Use when matching MS/MS spectra, identifying metabolites, or lib

Applies medicinal-chemistry filters with the medchem library — drug-likeness rules (Lipinski, Veber), PAINS filters, structural alerts, and molecular complexity metrics for compound prioritization and library cleanup. Use when filtering or triaging a compound library, flagging PA

Featurizes molecules for machine learning with molfeat — ECFP/MACCS/MAP4 fingerprints, RDKit and Mordred physicochemical descriptors, pharmacophore and shape descriptors, and pretrained embeddings (ChemBERTa, ChemGPT, CheMeleon) exposed as scikit-learn transformers that convert S

Queries the Precision Medicine Knowledge Graph (PrimeKG) for multiscale biomedical relationships across genes, drugs, diseases, phenotypes, pathways, and biological processes. Use when exploring drug-disease or gene-disease links, building disease-centric knowledge subgraphs, or

Loads Therapeutics Data Commons (TDC, PyTDC) AI-ready drug-discovery datasets and benchmarks — ADME, toxicity, drug-target interaction (DTI), scaffold splits, and molecular oracles for therapeutic ML and pharmacological prediction. Use when fetching a standardized benchmark datas

Every VM came back. The cluster did not. Declarative systems converge on config, and the datapath isn't config.

A surprising share of AI-in-the-terminal failures aren't the AI. They're zsh, and a version of bash from 2006.

A Claude Code plugin turns standalone project configuration into a namespaced, installable extension that teams and communities can update as one unit.

None of the safety came from the model. It came from six boring habits.

Skills package instructions and references. Subagents run work in a separate context and return results. They solve different problems and can be composed deliberately.

Six hours in, one step left, everything green, and the incident that didn't happen

CLAUDE.md carries persistent project context. Skills load reusable procedures when relevant. Separating stable facts from task-specific workflows keeps both easier to maintain.

Twenty minutes recovering secrets that never existed, and the one sentence from a human that ended it

An API request routing a model's tool call through an approval gate to a remote MCP server

31 config keys, two audits, and why the first one was wrong in both directions

The official MCP Registry stores standardized server metadata rather than package code. Publishers verify a namespace, describe installation or remote access, and submit immutable versions.

Everyone looks at the Dockerfile. The file that actually leaked the key was the project file.

Remote MCP authorization uses established OAuth standards, but secure integration still requires issuer validation, least-privilege scopes, protected token handling, and server-side enforcement.

"Copy it over and switch the reference" is two steps, and the outage lives in the one nobody checks

stdio fits local processes and prototypes. Streamable HTTP fits hosted services and shared integrations. The right choice follows where the capability runs and who must reach it.

The most important rule wasn't about what I could change. It was about what I was allowed to display.

Tools perform operations, resources expose readable context, and prompts provide reusable templates. Choosing the correct primitive makes an MCP server easier to understand and govern.

Use MCP Inspector to connect to local or remote servers, inspect capabilities, call tools, read resources, test prompts, and diagnose failures before release.

Build an MCP server in TypeScript with focused tools, validated schemas, local and remote transports, Inspector tests, and production security controls.

An MCP server exposes tools, resources, or prompts through a standard protocol so an AI application can discover and use external capabilities.

Create a project

Find what is safe to remove

Check what is safe to publish

Test yourself on your own pages

Rename a page and fix every link

Write a research report

Fix a page's type

Periodic review of what the vault learned

Undo the last run

Set up scheduled maintenance

Check frontmatter against the schema

Pull knowledge into a project

Scan for credentials

Show what a claim rests on

Split an overloaded page

Find concept pages nobody has touched

Audit the tag vocabulary

How my sources developed over time

Show which pages an answer used

Add relation types where they matter

AI assistant in Telegram that remembers everything and helps you run your life. Self-hosted in one command.

知更 — 本地 AI 的上下文与记忆层。Mac 上用语音输入、情境代回并调度 Codex / Claude Code；iOS 正在成为随身记忆终端和本地 Agent 遥控器。Local-first · BYOK.

Agent skill that turns Claude Code / Codex into a motion-design studio for voiceover-driven explainer videos — word-level voiceover sync, 78 motion recipe cards…

CoCo Super Intelligence is the orchestration layer that turns Claude Code, Cursor, or Codex into an engineering department: a routed advisory board, 185 skills,…

Deploy AI agent teams at scale without compromise

大师.skill — 输入行业，自动调研 6 轨[行业大佬 / 工具地图 / 工作流 / 知识正典 / 信息源 / 术语标准] → 提炼为可运行的行业 Master OS skill；装到任意 Claude Code / OpenClaw / Codex / Hermes agent 即让其进入「这一行的资深人」模式。…

Notion MCP server for Claude, Cursor, ChatGPT & Claude Desktop. Connect AI agents to Notion via Model Context Protocol — pages, databases, blocks, comments, fil…

Hook-based token compressor for 5 AI CLI hosts (Claude Code, Copilot CLI, OpenCode, Gemini CLI, Codex CLI). Up to 95% bash compression, signature-mode for code…

Model-neutral agent desktop/runtime for private, enterprise, and OpenAI-compatible / Anthropic-compatible model API. Tested on DeepSeek, Qwen, Kimi, GLM models.…

AI agent skill for end-to-end hotel search and booking—compare live rates across leading OTAs and hotel suppliers, verify availability, book stays, and manage r…

More than just Karpathy’s LLM Wiki, 100% local with Ollama. Drop Markdown notes → AI extracts concepts → your Obsidian wiki auto-links and grows. Zero sharing.…

Claude Code Guide - Setup, Commands, workflows, agents, skills & tips-n-tricks from beginner to power user!

A magical tool that changes how you use Agents. Install once — every Agent automatically discovers and uses all your MCP tools, and saves your tokens along the…

Open-source 24/7 Cowork app for OpenClaw, Hermes, Claude Code, Codex, OpenCode and 20+ more CLI Agent | Customize your assistants | Team them up｜Star if you lik…

AgenticX is a unified, production-ready multi-agent platform — Python SDK + CLI (agx) + Studio server + Machi desktop app. Features Meta-Agent orchestration, 15…

Query, provision and operate Cloud, SaaS, API and Model Context Protocol (MCP) resources through a unified SQL-based framework for humans and AI agents.

Open-source AI job search: scan job portals, evaluate listings into a structured A-H report with a global 1-5 score, tailor your CV, track applications — runs l…

A self-healing scraper for hostile sites: broken selectors repair themselves, browser rendering kicks in when needed, and a coherent identity layer (Chrome TLS…

Lightweight AI-native workflow builder for individuals and small teams — describe your idea in natural language, get a runnable workflow on a visual canvas, pub…

Minimal AI coding agent (~1,000 lines of Python) inspired by Claude Code. Works with any LLM. Think NanoGPT for coding agents. Formerly NanoCoder.