LLM Mart Basic

Run ESM protein language models — ESMC for embeddings and representations, ESMFold2 for structure prediction, and ESM3 for generative multimodal protein design across sequence, structure, and function — locally or through the hosted Biohub Platform API (formerly Forge). Use when

Manipulate, annotate, and render phylogenetic trees programmatically with the ETE Toolkit (ete3) — parse and edit Newick/NHX, detect duplication/speciation events, infer orthology and paralogy, query NCBI taxonomy, and export PDF/SVG figures. Use when traversing or reformatting t

Parse and write FCS (Flow Cytometry Standard) files v2.0-3.1 with FlowIO — extract event data as NumPy arrays, read $-keyword metadata and channel/parameter definitions, and convert events to CSV or pandas DataFrame. Use when loading raw .fcs flow-cytometry files, inspecting chan

Run fast one-liner queries to 20+ bioinformatics databases from the gget CLI or Python — gene info (Ensembl), BLAST, AlphaFold structures, Enrichr enrichment, and more. Use for quick interactive lookups of genes, sequences, structures, or pathways — for batch processing or advanc

Analyze and engineer protein glycosylation — scan sequences for N-glycosylation sequons (N-X-S/T), predict O-glycosylation hotspots, and reach curated glycoengineering tools (NetOGlyc, GlycoShield, GlycoWorkbench). Use when identifying or designing glycosylation sites, optimizing

Extract and preprocess tiles from whole-slide images (WSI) with histolab — OpenSlide-backed slide loading, tissue detection and masks, Random/Grid/Score tile extraction, and image/morphological filters for H&E preprocessing. Use when the user needs lightweight WSI slide preproces

Manage, annotate, and trace biological data with LaminDB, an open-source FAIR data framework that makes datasets queryable, versioned, and reproducible. Use when registering or querying biological datasets (scRNA-seq, spatial, flow cytometry), validating and curating data against

Design protein sequences around bound ligands, metals, and nucleic acids with LigandMPNN (Dauparas 2023) — inverse folding that conditions on non-protein context, so binding-pocket and metal-site residues are chosen to fit the actual ligand. Use when designing a small-molecule or

Analyze Neuropixels 1.0/2.0 extracellular electrophysiology with SpikeInterface — load SpikeGLX/Open Ephys recordings, preprocess and motion-correct, run Kilosort4 spike sorting, compute quality metrics, apply Allen/IBL curation, and do AI-assisted visual inspection. Use when wor

Runs FASTQ-to-VCF germline and somatic variant calling via the Nextflow nf-core/sarek pipeline pinned to -r 3.10.0 — builds the samplesheet.csv (patient, sex, status, sample, lane, fastq_1, fastq_2), runs bwa-mem/bwa-mem2/dragmap alignment plus GATK4 MarkDuplicates and BQSR again

Run full computational-pathology workflows with PathML — whole-slide-image (WSI) analysis across 160+ slide formats, multiplexed immunofluorescence (CODEX, Vectra, MERFISH), nucleus segmentation/classification (HoVer-Net, HACTNet), tissue- and cell-graph construction, HDF5 datase

Build phylogenetic trees end-to-end from raw sequences — MAFFT multiple sequence alignment, optional TrimAl trimming, IQ-TREE 3 maximum-likelihood inference with model selection and bootstraps, FastTree for large datasets, then visualize with ETE3 or FigTree. Use when reconstruct

Design protein sequences for a fixed backbone with ProteinMPNN (Dauparas 2022) — message-passing inverse folding that outputs sequences predicted to fold to a given structure, with fixed positions, tied/symmetric chains, amino-acid bias, and a soluble-model variant. Use when inve

Run differential gene expression analysis on bulk RNA-seq count matrices with PyDESeq2, the Python port of DESeq2 — size-factor normalization, dispersion estimation, Wald tests, FDR (Benjamini-Hochberg) correction, and volcano/MA plots. Use when identifying differentially express

Build complete mass-spectrometry workflows with pyOpenMS — feature detection, peptide identification, protein quantification, and full LC-MS/MS pipelines across many MS file formats (mzML, mzXML) and algorithms. Use for comprehensive proteomics and MS data processing — for simple

Read and write genomic alignment and variant files in Python with pysam (htslib bindings) — SAM/BAM/CRAM alignments, VCF/BCF variants, and FASTA/FASTQ sequences, plus region extraction and per-base coverage/pileup. Use when scripting NGS data-processing pipelines that parse, filt

Runs 16S/ITS amplicon (microbiome) analysis with the QIIME 2 distribution (2026.7; the "amplicon" distribution was renamed "qiime2" in 2026.4) in the correct order: manifest import, cutadapt trim-paired primer removal BEFORE dada2 denoise-paired (trunc-len chosen from the demux q

Generate de-novo protein backbones with RFdiffusion (Watson 2023) — a diffusion model for unconditional monomer generation, motif scaffolding, binder design against a target, and symmetric oligomers. Use when generating a new protein backbone from scratch, scaffolding a functiona

Quantifies bulk RNA-seq transcript abundance with salmon 2.x (the Rust rewrite; selective alignment or --sketch) and kallisto (v0.52.0, kb-python workflow), builds a decoy-aware gentrome index, runs quant with --gcBias -l A, then imports estimates via tximport/tximeta with a tx2g

Run the standard single-cell RNA-seq analysis pipeline with Scanpy on AnnData — QC filtering, normalization, dimensionality reduction (PCA, UMAP, t-SNE), Leiden/Louvain clustering, marker/differential expression, PAGA trajectories, and plotting. Use when analyzing scRNA-seq data

Discovery worked. Ping worked. Every TCP connection timed out, and later the tunnel only worked when someone had a terminal open.

Every VM came back. The cluster did not. Declarative systems converge on config, and the datapath isn't config.

A surprising share of AI-in-the-terminal failures aren't the AI. They're zsh, and a version of bash from 2006.

A Claude Code plugin turns standalone project configuration into a namespaced, installable extension that teams and communities can update as one unit.

None of the safety came from the model. It came from six boring habits.

Skills package instructions and references. Subagents run work in a separate context and return results. They solve different problems and can be composed deliberately.

Six hours in, one step left, everything green, and the incident that didn't happen

CLAUDE.md carries persistent project context. Skills load reusable procedures when relevant. Separating stable facts from task-specific workflows keeps both easier to maintain.

Twenty minutes recovering secrets that never existed, and the one sentence from a human that ended it

An API request routing a model's tool call through an approval gate to a remote MCP server

31 config keys, two audits, and why the first one was wrong in both directions

The official MCP Registry stores standardized server metadata rather than package code. Publishers verify a namespace, describe installation or remote access, and submit immutable versions.

Everyone looks at the Dockerfile. The file that actually leaked the key was the project file.

Remote MCP authorization uses established OAuth standards, but secure integration still requires issuer validation, least-privilege scopes, protected token handling, and server-side enforcement.

"Copy it over and switch the reference" is two steps, and the outage lives in the one nobody checks

stdio fits local processes and prototypes. Streamable HTTP fits hosted services and shared integrations. The right choice follows where the capability runs and who must reach it.

The most important rule wasn't about what I could change. It was about what I was allowed to display.

Tools perform operations, resources expose readable context, and prompts provide reusable templates. Choosing the correct primitive makes an MCP server easier to understand and govern.

Use MCP Inspector to connect to local or remote servers, inspect capabilities, call tools, read resources, test prompts, and diagnose failures before release.

Build an MCP server in TypeScript with focused tools, validated schemas, local and remote transports, Inspector tests, and production security controls.

Extract facts from meetings and update your knowledge base — person profiles, chronological log, and index. Use when the user asks "ingest my meetings", "update my knowledge base", "extract facts from meetings", "sync meetings to wiki", "backfill knowledge", or wants their PARA/Obsidian/wiki profiles updated from conversation data.

Health-check your meeting knowledge for contradictions, stale commitments, and decision conflicts. Use when the user asks "any conflicts in my meetings", "check for stale action items", "lint my meetings", "consistency check", "are there contradictions", or wants to audit their decision history.

List recent meetings and voice memos. Use when the user asks "what meetings did I have", "show my recent recordings", "any meetings today", "list my voice memos", or wants an overview of their meeting history. Also use when they need to find a specific meeting by browsing rather than searching.

Act as the user's live meeting sidekick inside the current terminal agent session. Use when the user explicitly asks you, the terminal agent, to watch a meeting, follow the live transcript, answer during the call, offer strategist thoughts, silently watch for risks, or track decisions. Do not use this skill to start or control the separate Minutes Coach HUD; explicit Coach or HUD lifecycle requests belong to minutes-copilot, and an ambiguous request such as "coach me live" requires one short surface clarification.

Self-coaching analysis of your own behavior across meetings — talk-time ratio, filler words, hedging language, monologue length, energy patterns, and (when meetings are tagged via /minutes-tag) what your behavior in winning meetings looks like vs losing ones. Use this whenever the user says "how did I do", "review my last meeting", "mirror", "self-review", "show my patterns", "coach me", "where am I weak", "talk time", "am I improving", "what do I do in meetings I win", "feedback on me", or asks for any kind of personal feedback on their own meeting behavior. This is the rare skill that gives the user a mirror to their own habits — surface it whenever they show curiosity about their own performance, even if they don't use the word "mirror".

Add a note to the current recording or annotate a past meeting. Use whenever the user says "note that", "remember this", "mark this as important", "add a note about", "annotate the meeting", or wants to capture a thought during or after a recording. Plain text input — no markdown needed.

Interactive meeting preparation — builds a relationship brief and talking points before a call. Use when the user says "prep me for my call with", "I'm meeting with X", "prepare me for", "what should I bring up with", "meeting prep", "get ready for my call", or wants to review history with someone before a meeting.

Generate a daily digest of today's policy-authorized meetings and voice memos — key decisions, action items, and themes across available recordings. Use when the user asks "recap my day", "what happened in my meetings today", "daily summary", "what did I discuss today", "any action items from today", or wants a consolidated view of the day's conversations.

Start or stop recording a meeting, call, or voice memo. Use this whenever the user says "record", "start recording", "capture this meeting", "stop recording", "I'm in a meeting", "take notes on this call", or wants to transcribe live audio. Also use when they ask about recording status or want to know if something is being recorded.

Draft user-facing Minutes release notes for a version from the commit range, recent GitHub releases, and the repository release checks. Use when the user asks to write, generate, prepare, revise, or review release notes or a changelog for a Minutes version.

Search past meeting transcripts and voice memos for specific topics, people, decisions, or ideas. Use this whenever the user asks "what did we discuss about X", "find that meeting where we talked about Y", "what did Alex say", "did we decide on", "what was that idea about", or any question that could be answered by searching their meeting history. Also use for "do I have any notes about" or "check my meetings for".

Guided first-time setup for Minutes — download whisper model, create directories, configure audio input. Use when the user says "set up minutes", "install minutes", "first time setup", "configure minutes", "get started with minutes", "how do I start using minutes", or when verify shows missing components.

Lightweight outcome tagging for meetings — won, lost, stalled, great, or noise. Use whenever the user says "tag this meeting", "mark that as a win", "that one was a loss", "tag yesterday's call as stalled", "mark this great", "that meeting was noise", "label that meeting", or any time they describe a meeting outcome in passing. Tagging takes 5 seconds and unlocks /minutes-mirror correlation analysis — the more meetings get tagged, the smarter mirror gets at telling the user what behavior patterns lead to wins. Surface this skill any time the user mentions a meeting result, win, loss, or wasted time.

Verify that Minutes is properly set up and working — model downloaded, mic accessible, directories exist, no stale state. Use when the user says "is minutes working", "check my setup", "verify minutes", "test recording setup", "why isn't minutes working", "minutes health check", or after running setup for the first time.

Analyze a product walkthrough, bug report video, Loom, or ScreenPal using Minutes transcription plus visual review. Use when the user wants a recorded demo or bug clip turned into a durable brief with transcript, key frames, issues, and next steps.

Weekly meeting synthesis — themes, decision arcs, stale commitments, and what deserves your attention next week. Use when the user says "weekly review", "what happened this week", "weekly summary", "recap my week", "any outstanding items", "week in review", or at the end of a work week.

Search agent history or trace code to its original agent session

Perform a non-destructive cross-artifact consistency and quality analysis across spec.md, plan.md, and tasks.md after task generation.

Generate a custom checklist for the current feature based on user requirements.

Identify underspecified areas in the current feature spec by asking up to 5 highly targeted clarification questions and encoding answers back into the spec.

Open-source super AI assistant & Agent Harness. Plans tasks, runs tools and skills, self-evolves with memory and knowledge. Multi-model, multi-channel. Lightwei…

Open-source AI browser agent for web automation: a web browsing agent and computer-use agent in plain English. Browser MCP for Claude Code and Gemini CLI.

Agent-native trading terminal built on DeepSeek Harness. Crypto, US, CN and HK in one three-column GUI, 19+ hot-swappable connectors, dry-run by default with hu…

Open-source desktop and web coding agent with a first-party host and harness, durable sessions, model connections, streaming chat, and workspace tools.

OrcaReplay — Time travel for AI agents. Record, replay, fork, and debug any agent run with any model. Built by the OrcaRouter.ai team.

A complete toolkit for connecting R and LLMs

OpenSRE — the memory-first AI SRE. Self-hosted incident investigation with episodic memory, knowledge graph, web console, Slack & Teams. opensre.in

A desktop AI coding assistant that works with your local projects. Ally helps you understand code, edit files, search a workspace, manage tasks, and complete de…

【在线免费使用】 简单快速将SQL或DBML转换为美观的ER图（支持 Agent Skill）/ The best SQL to ER Diagram converter (Support Agent Skill).

Operation-bound protection for autonomous AI agents: contain and verify operations before committing changes, understand intent and risk across long-running wor…

Local-first AI coding agent desktop: Electron + Rust host core + pi Agent Harness + user-installable plugins

Local-only Go static analysis engine with a built-in MCP server. Gives AI coding agents deterministic structural awareness: call graphs, impact analysis, symbol…

Self-hosted AI agent workspace with tool calling, MCP, multi-model routing, sandboxed execution, multi-agent workflows, and LLM-authored 3D character animation…

Automated TDD enforcement for Claude Code

High-performance platform for building websites, e-commerce, and web applications—with Native AI, JavaScript development, and a marketplace for portable sites a…

Autonomous AI-agent orchestration engine for job discovery with decision traces, tool adapters, and production-grade run control.

Token efficient Claude Code full Python rebuild. AI Coding Agent in 310K LoC Python.

OPC — One Person Company. A full team in a single Claude Code skill. Adaptive agent orchestrator with 21 built-in roles, 6 flow templates, and adversarial quali…

Open-source, secure environment with real-world tools for enterprise-grade agents.

MCP server for AI-powered GitHub project management — agent orchestration, PRD-to-issues pipeline, sprint planning, and multi-agent swarm coordination