LLM Mart Basic

Query the CZ CELLxGENE Census (200M+ cells) programmatically via cellxgene-census and TileDB-SOMA, slicing expression by tissue, disease, or cell type and returning AnnData. Use when pulling reference single-cell RNA-seq data from the largest curated public atlas, running populat

Predict biomolecular complexes with Chai-1, an open AlphaFold3-style model that folds multi-entity assemblies (proteins, ligands, nucleic acids) from a single typed FASTA — strong on antibody–antigen and protein–ligand complexes, with optional MSA and restraint inputs. Use when p

Build and analyze genome-scale constraint-based metabolic models with COBRApy — flux balance analysis (FBA), flux variability analysis (FVA), gene and reaction knockouts, flux sampling, and SBML model I/O. Use when simulating metabolic networks, predicting growth or knockout phen

Process and visualize deep-sequencing coverage with the deepTools CLI — convert BAM to bigWig (bamCoverage), build log2 ratio tracks (bamCompare), run QC (multiBamSummary correlation, PCA, plotFingerprint), apply the ATAC-seq Tn5 shift (alignmentSieve --ATACshift), and make TSS/p

Run ESM protein language models — ESMC for embeddings and representations, ESMFold2 for structure prediction, and ESM3 for generative multimodal protein design across sequence, structure, and function — locally or through the hosted Biohub Platform API (formerly Forge). Use when

Manipulate, annotate, and render phylogenetic trees programmatically with the ETE Toolkit (ete3) — parse and edit Newick/NHX, detect duplication/speciation events, infer orthology and paralogy, query NCBI taxonomy, and export PDF/SVG figures. Use when traversing or reformatting t

Parse and write FCS (Flow Cytometry Standard) files v2.0-3.1 with FlowIO — extract event data as NumPy arrays, read $-keyword metadata and channel/parameter definitions, and convert events to CSV or pandas DataFrame. Use when loading raw .fcs flow-cytometry files, inspecting chan

Run fast one-liner queries to 20+ bioinformatics databases from the gget CLI or Python — gene info (Ensembl), BLAST, AlphaFold structures, Enrichr enrichment, and more. Use for quick interactive lookups of genes, sequences, structures, or pathways — for batch processing or advanc

Analyze and engineer protein glycosylation — scan sequences for N-glycosylation sequons (N-X-S/T), predict O-glycosylation hotspots, and reach curated glycoengineering tools (NetOGlyc, GlycoShield, GlycoWorkbench). Use when identifying or designing glycosylation sites, optimizing

Extract and preprocess tiles from whole-slide images (WSI) with histolab — OpenSlide-backed slide loading, tissue detection and masks, Random/Grid/Score tile extraction, and image/morphological filters for H&E preprocessing. Use when the user needs lightweight WSI slide preproces

Manage, annotate, and trace biological data with LaminDB, an open-source FAIR data framework that makes datasets queryable, versioned, and reproducible. Use when registering or querying biological datasets (scRNA-seq, spatial, flow cytometry), validating and curating data against

Design protein sequences around bound ligands, metals, and nucleic acids with LigandMPNN (Dauparas 2023) — inverse folding that conditions on non-protein context, so binding-pocket and metal-site residues are chosen to fit the actual ligand. Use when designing a small-molecule or

Analyze Neuropixels 1.0/2.0 extracellular electrophysiology with SpikeInterface — load SpikeGLX/Open Ephys recordings, preprocess and motion-correct, run Kilosort4 spike sorting, compute quality metrics, apply Allen/IBL curation, and do AI-assisted visual inspection. Use when wor

Runs FASTQ-to-VCF germline and somatic variant calling via the Nextflow nf-core/sarek pipeline pinned to -r 3.10.0 — builds the samplesheet.csv (patient, sex, status, sample, lane, fastq_1, fastq_2), runs bwa-mem/bwa-mem2/dragmap alignment plus GATK4 MarkDuplicates and BQSR again

Run full computational-pathology workflows with PathML — whole-slide-image (WSI) analysis across 160+ slide formats, multiplexed immunofluorescence (CODEX, Vectra, MERFISH), nucleus segmentation/classification (HoVer-Net, HACTNet), tissue- and cell-graph construction, HDF5 datase

Build phylogenetic trees end-to-end from raw sequences — MAFFT multiple sequence alignment, optional TrimAl trimming, IQ-TREE 3 maximum-likelihood inference with model selection and bootstraps, FastTree for large datasets, then visualize with ETE3 or FigTree. Use when reconstruct

Design protein sequences for a fixed backbone with ProteinMPNN (Dauparas 2022) — message-passing inverse folding that outputs sequences predicted to fold to a given structure, with fixed positions, tied/symmetric chains, amino-acid bias, and a soluble-model variant. Use when inve

Run differential gene expression analysis on bulk RNA-seq count matrices with PyDESeq2, the Python port of DESeq2 — size-factor normalization, dispersion estimation, Wald tests, FDR (Benjamini-Hochberg) correction, and volcano/MA plots. Use when identifying differentially express

Build complete mass-spectrometry workflows with pyOpenMS — feature detection, peptide identification, protein quantification, and full LC-MS/MS pipelines across many MS file formats (mzML, mzXML) and algorithms. Use for comprehensive proteomics and MS data processing — for simple

Read and write genomic alignment and variant files in Python with pysam (htslib bindings) — SAM/BAM/CRAM alignments, VCF/BCF variants, and FASTA/FASTQ sequences, plus region extraction and per-base coverage/pileup. Use when scripting NGS data-processing pipelines that parse, filt

A surprising share of AI-in-the-terminal failures aren't the AI. They're zsh, and a version of bash from 2006.

A Claude Code plugin turns standalone project configuration into a namespaced, installable extension that teams and communities can update as one unit.

None of the safety came from the model. It came from six boring habits.

Skills package instructions and references. Subagents run work in a separate context and return results. They solve different problems and can be composed deliberately.

Six hours in, one step left, everything green, and the incident that didn't happen

CLAUDE.md carries persistent project context. Skills load reusable procedures when relevant. Separating stable facts from task-specific workflows keeps both easier to maintain.

Twenty minutes recovering secrets that never existed, and the one sentence from a human that ended it

An API request routing a model's tool call through an approval gate to a remote MCP server

31 config keys, two audits, and why the first one was wrong in both directions

The official MCP Registry stores standardized server metadata rather than package code. Publishers verify a namespace, describe installation or remote access, and submit immutable versions.

Everyone looks at the Dockerfile. The file that actually leaked the key was the project file.

Remote MCP authorization uses established OAuth standards, but secure integration still requires issuer validation, least-privilege scopes, protected token handling, and server-side enforcement.

"Copy it over and switch the reference" is two steps, and the outage lives in the one nobody checks

stdio fits local processes and prototypes. Streamable HTTP fits hosted services and shared integrations. The right choice follows where the capability runs and who must reach it.

The most important rule wasn't about what I could change. It was about what I was allowed to display.

Tools perform operations, resources expose readable context, and prompts provide reusable templates. Choosing the correct primitive makes an MCP server easier to understand and govern.

Use MCP Inspector to connect to local or remote servers, inspect capabilities, call tools, read resources, test prompts, and diagnose failures before release.

Build an MCP server in TypeScript with focused tools, validated schemas, local and remote transports, Inspector tests, and production security controls.

An MCP server exposes tools, resources, or prompts through a standard protocol so an AI application can discover and use external capabilities.

Treat an AI agent skill as both an instruction package and a software dependency: inspect what it says, what it runs, what it can access, and how it updates.

Turn a GAIA workflow misfire into a redacted, classified, filing-ready bug report in one read-only pass. Self-diagnoses config issues inline; files probable bugs upstream on confirmation.

Judge-the-form, human-gated hardening. Reviews recurring code-audit-frontend findings and, with approval, drafts the lowest-context-weight form (deterministic check / skill / path-scoped prose rule) into the working tree. Pass `list` to see live candidates or `why <finding_class>` to explain one.

Initialize a new project from the GAIA React template, renames, strips GAIA branding, configures i18n, installs Claude skills/plugins.

Plan a complex feature using GAIA's task-orchestration pattern, structures the work into fresh-context subagent phases for your approval. Does not implement.

Cut a new GAIA release, bump version, graduate CHANGELOG, regenerate manifest, open release PR, then tag on merge. Maintainer-only.

Detect and, on explicit consent, additively append the languages Serena is not indexing to the `languages:` list in `.serena/project.yml`, then prompt a Serena restart. Never mutates without a yes; inert without Serena.

Author an immutable SPEC artifact through Socratic discovery (spec-kit wrapper), then STOP. Terminal, never runs /gaia-plan; it prints a /gaia-plan prompt the human pastes into a fresh session. Pass `auto <description>` for non-interactive mode that answers its own questions.

Maintainer-only autonomous health audit + auto-heal loop. Runs N=3 fresh-team audit-fix-audit cycles with circuit breakers, reports an F-to-A+ verdict (folding in the shared Claude-integration fitness grade) or escalates.

Single post-init onboarding command; detects situation, runs only owed phases; safe to re-run. --reconfigure rotates token and re-selects tools.

GAIA before_specify hook: constitution placeholder check + spec-kit version-pin drift detection.

GAIA after_specify hook: immutability lint over the just-written SPEC artifact.

Close a plan after implementation+merge. Offers wiki-promote for the plan's consolidated SUMMARY.md, cold-consolidates an out-of-band merge, then early-reaps the local plan folder once cost is represented in cost.jsonl.

GAIA self-review: pre-gate-2 review pass on the in-progress SPEC draft.

Close a SPEC after implementation+merge. Optional drain of deferred wiki-promote, cold-consolidates an out-of-band merge into SUMMARY.md, then early-reaps the local SPEC folder once cost is represented in cost.jsonl.

GAIA Socratic discovery wrapper: /speckit-specify for the initial draft, then GAIA's own Socratic clarify loop.

GAIA before_implement hook: render PO-authored UATs into Playwright e2e specs at .playwright/e2e/spec-NNN/.

Promote merged SPEC or plan content into the GAIA wiki.

This project uses the GAIA preset. Bare `/speckit-clarify` is not the clarify path here: core clarify writes an off-shape artifact (a `## Clarifications` / `### Session` block with five-word answers) and carries a question cap GAIA does not use. Run `/gaia-spec` instead — it driv

GAIA-wrapped /speckit-specify: writes through core, then relocates the artifact to .gaia/local/specs/SPEC-NNN/SPEC.md and stamps GAIA frontmatter.

Show or configure the compact Fallow Impact statusline in Claude Code

AI coding platform for teams

AI Multi-Agent Framework in .NET

Production-ready code examples for Telnyx AI Communications Infrastructure — Voice AI, SMS, SIP, and IoT APIs

UltraGameStudio - AI coding agent for game development: engine workflows, gameplay code, and asset generation.

🦞 ResearchClawBench: Evaluating AI Agents for Automated Research from Re-Discovery to New-Discovery

The context-aware model router that learns & adapts to your long-horizon coding agent workflows. Lightweight & extensible, works with any harnesses, any models,…

Open-source sandboxes where coding agents build and deploy. Spin up isolated environments where Claude Code, Cursor, and other agents code and deploy software.

Native desktop UI for Claude Code with orchestration, streaming, background agents, and multi-provider support. Built with Tauri + React.

OpenCode mobile client via Telegram: run and monitor AI coding tasks from your phone while everything runs locally on your machine. Scheduled tasks support.

Terminal session manager for AI coding agents. One TUI for Claude, Gemini, OpenCode, Codex, and more.

Exposes internet search tools for use by LLM-backed Assist in Home Assistant

Templates and workflow for generating PRDs, Tech Designs, and MVP and more using LLMs for AI IDEs

Versus Incident is the self-hosted AI SRE agent. It learns what your system normally look like and escalates only what is new or unexpected issues — routing to…

Browser-native side panel for Hermes Agent — connect web context to your local Hermes runtime.

Agentic-friendly CLI generator for APIs: turn Swagger, OpenAPI, and google.api.http protos into single-binary CLIs with catalogs and generated Skills.

Open-source agent platform for Global × China enterprises — wire every system through one agent core. Self-hosted, any LLM.

Official python implementation of UTCP. UTCP is an open standard that lets AI agents call any API directly, without extra middleware.

An agent small enough to run anywhere. A minimal agentic runtime in C — ~380 lines, 54KB binary, ~2MB RAM. A skill file + an LLM + a shell loop, no framework.

AgentiLoop Agent! An Autonomous Agentic Agent for Mac, and exclusive Apple only harnesss. Suppprtd automation, scripting, coding, build anything and more. Power…

微信公众号 AI 运营助手 | 选题、写稿、审稿、排版、配图、发布全流程 Skill，支持 OpenClaw / Claude Code / Cursor / Codex